David B. Brunson, DVM, MS, DACVA
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Published results can be found here.
Malignant Hyperthermia in Dogs: our research lab has identified a casual mutation for MH in a family of dogs. Roberts MC, Mickelson JR, Patterson EE, Nelson TE, Armstrong J, Brunson, DB, Hogan K. Autosomal dominant canine malignant hyperthermia is caused by mutation in the gene encoding the skeletal muscle calcium release channel (RYR1).
Anesthesiology 2001 Sep; 95(3):716-725
We are now attempting to locate this mutation in unrelated dogs. In order to do this we are seeking blood or muscle samples from dogs that exhibit clinical signs of Malignant Hyperthermia while anesthetized with gas anesthetics. The clinical signs of K9MH are as follows: increased expired CO2 > 65 mmHg, elevated body temperature > 106°F, arrhythmias and death if the anesthetic is not discontinued.
The progression in dogs is not as rapid as seen in pigs and people. Muscle rigidity and metabolic acidosis is frequently not seen. In many cases where the duration of anesthesia is less than 2 hours, only hypercapnia and hyperthermia will be observed. If you believe you have a case that fits the above clinical picture please contact me by email.
For a limited time our lab will isolate DNA and analyze it for the T1640C mutation at no charge. A report will be sent to the RDVM with the results of the analysis. Samples required for the analysis are as follows: > 5 mls of EDTA whole blood &/or > 2 cubic centimeters of skeletal muscle. Muscle samples should be wrapped in tin foil and frozen. Blood and muscle should be kept frozen and should be shipped on Dry Ice. Shipping time is not critical if the samples are kept frozen.
Pending funding, our lab may be able to screen MH suspect animals for other known human or pig mutations, if they do not have the T1640C mutation. Note: Our lab will not do phenotype muscle contracture testing of suspect MH dogs.
Thanks for your interest and support.
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Reviewed 5/10/04